Synthetic biology is in the midst of branching out from bacterial to eukaryotic systems. With the increased complexity of the eukaryotic cell come new challenges, but more importantly new ...

Transcription is the first step of gene expression, where a DNA sequence is copied into messenger RNA. In eukaryotes, transcription initiation starts with the assembly of the transcription ...

Interaction between proteins plays a vital role in several biological mechanisms. The yeast two-hybrid system is used to identify a large number of protein interactions in vivo. The standard ...

Chromatin remodeling is an important mechanism of regulating eukaryotic gene expression, which makes tightly condensed DNA accessible to various regulatory factors, such as transcription factors and ...

The regulation of mRNA transcription plays a key role in relaying signals from the environment and during development into the gene expression program of a cell. During transcription initiation, a ...

Although the DNA and its double-helix are one of the most familiar molecules of our time, our knowledge of how cells control what genes they want to express still is rather limited. In order to create ...