During the isolation of rose petal protoplasts, two successive transient abscisic acid (ABA) accumulations were detected when digest medium and tissue slices were assayed simultaneously, using ...
Protoplasts from suspension-cultured cells of Nicotiana glutinosa L. were lysed in 0.3 molar sorbitol in 2 millimolar ethylenediaminetetraacetate-tris(hydroxymethyl) aminomethane (pH 7.5) to release ...
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