This protocol describes the use of sortase-mediated reactions to label the N terminus of any given protein of interest. The sortase recognition sequence, LPXTG (for Staphylococcus aureus sortase A) or ...

Researchers at the U.S. Department of Energy's Argonne National Laboratory and the University of Chicago have determined the crystal structure of sortase B, an enzyme found in the bacteria that cause ...

Tracking interactions between different types of cells in the body can offer clues to an organism’s development, reveal cellular functions, or track tissues’ responses to treatments. However, existing ...

One of the goals of protein engineering is the installation of desirable features, template-encoded or otherwise, on proteins that naturally lack them. The ability to confer different functionalities ...