In a typical experiment to measure the Ka of a weakly binding ligand, for example, sufficient weak ligand is added to the target macromolecule to occupy approximately 50% of the binding sites.

This lab includes a demonstration of the proper use of a burette system for titrations; including a detailed description, instructional video, and opportunity for students to collect data to ...

ANTIBODY TITRATION is critical to the success of any flow cytometry experiment. This is even more so in microflow cytometric analysis of extracellular vesicles (EVs), where removing excess antibodies ...

Manual titrations can be extremely challenging and frustrating, impeding your efficiency in the lab. More importantly, these issues can lead to inconsistent results, waste valuable time, and stop ...

Center: Representative raw ITC data from titration experiment. Right: Representative binding isotherm from ITC experiment, fit to one set of sites binding model.

This protocol from Brautigam et al. describes methods for baseline correction and global analysis of isothermal titration calorimetry data using NITPIC and SEDPHAT. Publication-quality graphs of ...

Here, we present a step-by-step protocol for carrying out a footprint titration experiment. We then describe how to quantify the resultant images to generate individual-site binding curves.

The app provides a multi-sensory perception of colour change observed in a titration experiment where a solution of known concentration is used to determine the concentration of an unknown solution.